pAAVdual-rNSE-EGFP

pAAVdual-rNSE-EGFP is used to produce AAV-rNSE-EGFP virus using novel AAVdual production system. In this plasmid, the regular single strand AAV genome with a rNSE promoter and an EGFP reporter is cloned into our novel Ad helper plasmid, mini-pHelper. AAV-rNSE-EGFP viruses can be generated by co-transfection of this plasmid with regular AAV helper (pRCap) plasmids, carrying AAV2 rep gene and different cap genes, without adding additional Ad helper plasmid to supply E2A, E4orf6 and VA RNA functions.
The rNSE promoter refers to the promoter region of the rat Neuron-Specific Enolase (NSE) gene. Neuron-Specific Enolase is an enzyme that is highly expressed in neurons and neuroendocrine cells.The rNSE promoter is a neuron-specific regulatory element that drives gene expression in neurons and neuroendocrine cells. It is a crucial tool in neuroscience research, gene therapy for neurological disorders, and the development of transgenic models for studying the nervous system. Its specificity to neurons makes it invaluable for targeting gene expression in studies focused on neuronal function, neurodevelopment, and neurodegenerative diseases.

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