Neomycin Sulfate, EP

- Overview
  - Neomycin Sulfate, EP is a broad-spectrum aminoglycoside antibiotic composed of related compounds including Neomycin A (neamine), Neomycin B (framycetin), Neomycin C, and a few minor compounds. Neomycin B is the most active, followed by C and A. The quantities of these components vary from lot-to-lot.
    Neomycin Sulfate, EP conforms to European Pharmacopoeia specifications.
    
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    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    1405-10-3
    
    Molecular Formula
    
    C23H46N6O13 ·3 H2SO4
    
    Molecular Weight
    
    908.88
    
    Appearance
    
    White or yellowish powder
    
    Solubility
    
    Ethanol: 0.10 mg/mL
    Methanol: 0.23 mg/mL
    Water: 50 mg/mL
    
    Other Properties
    
    Source: Streptomyces fradiae
    PH: 5.0-7.5
    Assay: (Dried Basis): ≥680IU/mg
    Loss on Drying: ≤8.0%
    Optical Rotation: +53.5° to +59.4°
    
    Storage
    
    2-8°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Neomycin is a broad-spectrum, however, it is mostly used against Gram-negative bacteria.
    
    Microbiology Applications: Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).
    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:
    
    Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
    Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
    
    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringins and inhibit growth of Enterobacteria and Clostridium bifermentans.
    
    Plant Biology Applications: Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium-mediated transformation
    
    Eukaryotic Cell Culture Applications: Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For complete additional information on your cell culture needs, please visit our cell-culture database.