Name: Neomycin B Sulfate, EvoPure®
Price: 409.00 USD

- Overview
  - Neomycin B Sulfate, EvoPure® is the highly purified sulfate salt of Neomycin B, which is the major component found in Neomycin. Neomycin B is ~90% more active than Neomycin A and ~65% more active than Neomycin C, its stereoisomer. Highly pure Neomycin compounds can be used in cell culture, upstream biopharma, and cancer research.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    4146-30-9
    
    Molecular Formula
    
    C23H46N6O13 · xH2SO4 (lot specific)
    
    Molecular Weight
    
    614.64 (Free base)
    
    Appearance
    
    White powder
    
    Solubility
    
    Soluble in water
    
    Other Properties
    
    Source: Streptomyces fradiae
    PH: 5.7-7.5
    Purity Level: ≥98.0%
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Neomycin is a broad-spectrum antibiotic, however, it is mostly used against Gram-negative bacteria.
    
    Microbiology Applications: Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).
    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:
    
    Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
    Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
    
    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringens and inhibit growth of Enterobacteria and Clostridium bifermentans.
    
    Plant Biology Applications: Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation.
    
    Eukaryotic Cell Culture Applications: Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For additional information for your cell culture needs, please visit our cell-culture database.