MTG-Blocker

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Irreversible alkylation of the active site cysteine. C102 has a molecular weight of about 510 Da and consists of an electrophilic warhead and a peptidic backbone. First, the MTGs active site cysteinyl residue attacks the warhead. A subsequent reaction leads to the release of a leaving group. Finally, the peptidic backbone is irreversibly attached to the MTG active site. As a consequence, the mass of the enzyme increases +482 Da due to the attached peptidic backbone.
    
    More Details
    
    Zedira
- Properties
  - Name
    
    BTG-Blocker
    
    Purification
    
    >90 % (214 nm)
    
    Storage
    
    store at -20°C, desiccate
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Class
    
    Inhibitor
    
    Reconstitution
    
    500 μM in 0.1% (v/v) DMSO / aqueous buffers.Pre-dissolve e.g. 10 mg (27 μmol) in 54 μl DMSO - dilute e.g. 5 μl of that stock solution (500 mM) with 4995 μl aqueous buffer to obtain a 500 μM solution.Diluted buffer solutions must be consumed within 2 hours.DMSO stock solutions can be stored at ambient temperature for 4 weeks.
- Applications
  - Application Description
    
    Blocking of enzymatic activity of microbial (bacterial) transglutaminase IC50 ~ 125 μM(Microbial Transglutaminase Assay Z009; Zedira, Germany)
- Reference
  - Rickert, M. et al. Protein Sci. 2016, 25, 442-455.
    Spycher, P.R. et al. ChemBioChem 2017, 18, 1923-7.

Datasheet

MSDS

MTG-Blocker

Background

More Details

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