ZediXclusive Microbial Transglutaminase Assay Kit

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      • Overview
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          Transglutaminases are a family of enzymes that catalyse the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains (Folk, J. E. and Cole, P. W., 1966). These enzymes catalyse the acyl transfer reaction between the γ-carboxyamide group of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine (Lorand L. et al., 1962). The resulting cross link is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.

      • Properties
        • Formulation
          (A1) ACTIVITY REAGENT 1: lyophilized TRIS buffer pH 6.0 containing Z-Gln-Gly, hydroxylamine and reduced glutathione, 3 vials
          (A2) ACTIVITY REAGENT 2: Hydroxylamine, 3 vials
          (S) STOP REAGENT: Hydrochloric acid [4% v/v], Iron (III) chloride, 3 vials
          (P1) MICROBIAL TRANSGLUTAMINASE (positive control): 3 vials
          P1 has to be stored at -20°C (shipment is possible at 4 – 8°C). A1, A2, S has to be stored at 4 – 8°C. The unopened reagents are stable until the expiration date printed on the box.

          * INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.Artikel in DLG-Lebensmittel – 06/2017 zur Bestimmung von Transglutaminase in Lebensmitteln (deutsch)

      • Applications
        • Application
          Activity Assay
      • Reference
        • Grossowicz et al., J. Biol. Chem. 1950, 187:111-125
          Rickert et al., Protein Sci. 2015, accepted manuscript (DOI: 10.1002/pro.2833)

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