ZediXclusive Microbial Transglutaminase Assay Kit

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Transglutaminases are a family of enzymes that catalyse the posttranslational modification of proteins by inserting an isopeptide bond within or between polypeptide chains (Folk, J. E. and Cole, P. W., 1966). These enzymes catalyse the acyl transfer reaction between the γ-carboxyamide group of peptide-bound glutamine residues and a variety of primary amines, particularly the ε-amino group of lysine (Lorand L. et al., 1962). The resulting cross link is of great significance, since it is highly stable and also resistant to mechanical and proteolytic degradation.
    
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    Zedira
- Properties
  - Formulation
    
    (A1) ACTIVITY REAGENT 1: lyophilized TRIS buffer pH 6.0 containing Z-Gln-Gly, hydroxylamine and reduced glutathione, 3 vials
    (A2) ACTIVITY REAGENT 2: Hydroxylamine, 3 vials
    (S) STOP REAGENT: Hydrochloric acid [4% v/v], Iron (III) chloride, 3 vials
    (P1) MICROBIAL TRANSGLUTAMINASE (positive control): 3 vials
    
    Storage
    
    P1 has to be stored at -20°C (shipment is possible at 4 – 8°C). A1, A2, S has to be stored at 4 – 8°C. The unopened reagents are stable until the expiration date printed on the box.
    
    * INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
- Applications
  - Application
    
    Activity Assay
- Reference
  - Grossowicz et al., J. Biol. Chem. 1950, 187:111-125
    Rickert et al., Protein Sci. 2015, accepted manuscript (DOI: 10.1002/pro.2833)

Datasheet

MSDS

ZediXclusive Microbial Transglutaminase Assay Kit

ZediXclusive Microbial Transglutaminase Assay Kit

Background

More Details

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