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Overview
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mRNA-Fect is a highly effective transient transfection reagent optimized for mRNA delivery to both attachment-depended and suspension-growing cells. mRNA-Fect is a synthetic amphiphilic polymer that is tailored for mRNA delivery after extensive testing of amphiphilic polymer libraries. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated mRNA into ~100 nm particles with a net positive charge. The complexation between the mRNA and mRNA-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. mRNA-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with this reagent. mRNA-Fect has been tested and found effective for plasmid DNA and siRNA delivery as well as in certain cell types. As with all transfection reagents, formulations of mRNA-Fect with polynucleotides may need to be optimized for specific cell types and transfection conditions.
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More Details
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Overview
Transfection of Attachment-dependent Cells with mRNA-Fect Transfecting attachment-dependent MCF-7, MDA-MB-436, and MDA-MB-231 cells with mRNA-Fect. A mRNA coding for a reporter protein (Green Fluorescent Protein, GFP) was used to assess the efficiency of mRNA expression. Typical GFP expression levels were visualized under fluorescent microscopy (top pictures). The mRNA expression levels were quantified by flow cytometry 72 hours after transfection and summarized as the percentage of cells positive for GFP (bottom graph). For comparison a leading lipofection reagent was used according to the manufacture’s instructions.
Transfection of Suspension-growing Cells with mRNA-Fect Transfecting suspension K562 and THP-1 cells with mRNA-Fect. A mRNA coding for a reporter protein (Green Fluorescent Protein, GFP) was used to assess the efficiency of mRNA expression. Typical GFP expression levels were visualized under fluorescent microscopy (top pictures). The mRNA expression levels were quantified by flow cytometry 72 hours after transfection and summarized as the percentage of cells positive for GFP (bottom graph). For comparison a leading lipofection reagent was used according to the manufacture’s instructions.
mRNA-Fect Transfection Reagent
mRNA-Fect Transfection Reagent
Catalog Number:
TR02108484RJH
Mfr. No.:
80-10; 80-20
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