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Overview
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The CRISP-Fect reagent is a highly effective transfection reagent optimized for ribonucleoprotein (RNP) delivery to both attachment-dependent and suspension-growing cells. CRISP-Fect is able to bind RNPs complexes of Cas9 endonuclease and sgRNA. Upon co-incubation, the transfection reagent forms 50-150 nm particles with RNPs that bear a net positive charge. The formation of complexes is performed in aqueous buffers without any organic solvents during preparation. CRISP-Fect is a non-integrating reagent and does not cause genetic changes on its own after treatment. CRISP-Fect has been confirmed to provide effective delivery of Cas9 RNPs in different cell types. Figure 1 shows typical delivery of RNPs to attachment-dependent cells (breast cancer cells) and suspension-growing cells (Jurkat T-cells). As with all transfection reagents, formulations of CRISP-Fect with the intended cargo may need to be optimized for specific cell types and transfection conditions.
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More Details
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Overview
Figure 1: A) Left. Transfection of Jurkat T-cells and breast cancer MDA-MB-436 cells using CRISP-Fect. sgRNA/Cas9 complexes (RNP) were formulated with indicated transfection reagents and added to the cells for 24 hours. RNP uptake was followed by using FITC-labeled Cas9. The results are summarized as arbitrary fluorescence units, indicating the average amount of RNPs delivered per cell. Right: Percentage of cells with RNPs delivered. B) Left. Transfection of Jurkat T-cells with RNPs formulated at different ratios of Cas9/sgRNA using CRISP-Fect also tracked by FITC-labelled Cas9 and summarized as arbitrary units. Right. Percentage of cells with RNPs delivered. Lipofectamine 2000 and jetOptimus were used as commercial references.
CRISP-Fect RNP Transfection Reagent
CRISP-Fect RNP Transfection Reagent
Catalog Number:
TR02108485RJH
Mfr. No.:
90-10; 90-20
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