All-Fect Kit - Broadly Acting Transfection Reagent for siRNA and pDNA Delivery

ALL-Fect Kit is recommended for better efficacy in hard-to-transfect cell types, and ALL-Fect Kit contains 1 vial of ALL-Fect and 1 vial of Trans-Booster.

The All-Fect Kit is a highly effective transfection reagent combination optimized for both mRNA and DNA delivery to both attachment-dependent and suspension-growing cells. It is based on synthetic amphiphilic polymers tailored primarily for siRNA and DNA delivery after extensive testing of polymer libraries. It can undergo multivalent interactions with mRNA or DNA and encapsulating into nanoparticles (~100 nm) appropriate for effective cellular uptake. This interaction occurs in aqueous buffer, obviating the need for organic solvents during preparation. The All-Fect Kit is a nonintegrating carrier, so that the genetic make-up of host cells is not altered after the treatment. As with all transfection reagents, formulations of All-Fect Kit with polynucleotides may need to be optimized for specific cell types and transfection conditions.

Benefits
• High transfection efficiency in the presence of serum.
• Effective delivery of pDNA molecules via a simple protocol that is ideal for scale-up and automation.
• Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
• Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.
• Possibility of using the same transfection reagent in animal models, leading to harmonized studies.

Please contact us at for specific academic pricing.

Background

ALL-Fect is a highly effective, broadly-acting transfection reagent for attachment dependent and independent cells. ALL-Fect is a synthetic amphiphilic polymer that is tailored for both siRNA and DNA delivery to a wide range of cells. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the ALL-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. ALL-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. ALL-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension-growing cells. As with all transfection reagents, formulations of ALL-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.