METAFECTENE® - DNA & RNA Transfection Reagent

- Overview
  - METAFECTENE® is a polycationic transfection reagent based on liposome technology. The specifically designed molecular structure "proton-sponge" of the cationic lipid ensures easy entry of DNA or RNA into cells by condensing DNA or RNA into compact structures (DNA or RNA-lipid complex).
    METAFECTENE® is provided as a ready-to-use solution. It shows no serum inhibition, which makes it a reagent of choice for transfecting sensitive cell lines.
    
    Highlights
    ·Suitable for DNA & RNA transfection
    ·Suitable for stable and transient transfection
    ·Suitable for adherend and suspension cells
    ·High transfection efficiency
    ·Free from serum inhibition
    ·Low cytotoxicity: transfection solution can generally remain on the cells
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1 year (from date of delivery)
    Assays: Up to 1500 (24 well) or up to 400 (6 well) per 1 ml reagent
    
    Storage
    
    4℃
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    Suitable for transient and stable transfection of mammalian cell lines with DNA (plasmids, bacmids), RNA (mRNA, miRNA and siRNA) and modified nucleic acids (antisense oligonucleotides).
    Application areas:
    ·The production of proteins, antibodies and virus (e.g. AAV, adenovirus, lentivirus)
    ·Cotransfectionens of various nucleic acids, genome editing (e.g. CRISPR/Cas9, CRISPR/Cpf1) and gene silencing (gene knockdown)
- Reference
  - "Endogenously induced DNA double strand breaks arise in heterochromatic DNA regions and require ataxia telangiectasia mutated and Artemis for their repair." Woodbine et al., 2011, Nucleic acids research, 39.16: 6986-6997
    "Macroautophagy proteins assist Epstein Barr virus production and get incorporated into the virus particles." Nowag et al., 2014, EBioMedicine, 1.2-3: 116-125
    "EBV reduces autophagy, intracellular ROS and mitochondria to impair monocyte survival and differentiation." Gilardini Montani et al., 2019, Autophagy, 15.4: 652-667

MSDS

Documents

Product Information and Protocol

Recommended applications, sorted by nucleic acid

METAFECTENE® - DNA & RNA Transfection Reagent

METAFECTENE® - DNA & RNA Transfection Reagent

Documents

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