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DOTAP - DNA Transfection Reagent

DOTAP - DNA Transfection Reagent

Catalog Number:

TR02135775BIO

Mfr. No.:

T010-10; T010-20; T010-50

Price:

$257

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Quantity:

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- Overview
  - DOTAP is a monocationic transfection reagent based on liposome technology, as the first-generation transfection reagents. DOTAP transfects nucleic acids in the presence and absence of serum.
    
    Highlights
    ·Suitable for DNA transfection
    ·Ready-to-use formulation
    ·Results are often adequate for purpose
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1 year (from date of delivery)
    Content: 1 mg/ml 1,2-Di-(9Z-octadecenoyl)-3-trimethylammoniumpropane methyl sulfate in water
    Assays: Up to 150 (6 well) or up to 75 (60 mm) per 1 ml reagent
    
    Storage
    
    4°C
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    DOTAP is suitable for transient and stable transfection of mammalian cell lines with DNA (plasmids, bacmids) and modified nucleic acids (antisense oligonucleotides).
- Reference
  - "Parallel Clamps and Polypurine Hairpins (PPRH) for Gene Silencing and Triplex‐Affinity Capture: Design, Synthesis, and Use." Aviñó et al., 2019, Current protocols in nucleic acid chemistry, 77.1: e78

Documents

Product Information and Protocol

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