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Overview
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This product is suitable for extracting high-purity RNA from FFPE samples. The obtained RNA can be used for clinical in vitro detection.
Principle
This product is based on silica gel column purification. The samples were digested by FRL and protease K to release RNA. After adding denatured solution FBD and ethanol, it is transferred to the column for filtration. RNA is adsorbed on the membrane of the column, while protein is not adsorbed and filtered out with the solution. Protein and salt are removed by washing the column, and finally the RNA was eluted by low salt buffer NW.
Advantages
• Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
• High quality - high purity total RNA can be directly used in various sensitive downstream applications
• Fast - several samples can be extracted in 60 minutes by column method
• Safe - no phenol chloroform extraction required
• Sensitive - RNA can be recovered at the level of PGPlease contact us at for specific academic pricing.
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- Properties
- Applications
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Overview