Human tissue transglutaminase, C230A mutant

- Overview
  - His6-rhTG2-Cys230Ala is based on the TGM2-allele from I.M.A.G.E.-clone IMAGp958L121020 isolated from neuroblastoma cells of the human brain (Val224-allele, Kanchan et al., Biochem. J. 2013, 455:261–72).It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MAHHHHHHAEELV. At position 230 Cysteine has been replaced by Alanine. Please note that numeration corresponds to the wild type protein without hexahistidine-tag. His6-rhTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95% purity.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    Tissue-type Transglutaminase, TG2, TGase 2, proteinglutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8.
    
    Storage
    
    Store at –20 °C in working aliquots. Repeated freezing and thawing is not recommended. Delivery at ambient temperature is possible.
    
    Note
    
    INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
    
    Molecular Weight
    
    78 kDa
    
    Class
    
    Human Transglutaminase 2
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be stored frozen in working aliquots. For short term storage keep cooled on ice.
- Applications
  - Application Description
    
    His6-rhTG2-Cys230Ala may be used for immunoprecipitation.
- Reference
  - Stamnaes, J. et al, JBC, 2010, 285:25402-9