Human keratinocyte transglutaminase

- Overview
  - His6-rhTG1 is based on the TGM1-allele from I.M.A.G.E.-clone IRAKp961M1628 isolated from human skin squamous cell carcinoma. It is N-terminally fused to a hexahistidine-tag resulting in the encoded N-terminal amino acid sequence MHHHHHHMDGPR. His6-rhTG1 is purified by IMAC to more than 90 % purity.
    
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    More Details
    
    Zedira
- Properties
  - Name
    
    TG1, TGase 1, keratinocyte protein-glutamine-γ-glutamyltransferase
    
    Source
    
    E. coli
    
    Type
    
    Recombinant Proteins
    
    Purification
    
    > 90 % (visually by SDS-PAGE)
    
    Formulation
    
    The Transglutaminase is lyophilized from 50 mM Tris-HCl pH 8.0, 10 mM Glutathion
    
    Storage
    
    Store at ≤ - 20°C. Store working aliquots at ≤ - 20°C. Avoid repeated freezing and thawing. Delivery at ambient temperature is possible
    
    Note
    
    Intended for research use only, not for use in human, therapeutic or diagnostic applications.
    
    Molecular Weight
    
    90 kDa
    
    Reconstitution
    
    Add the volume of water specified in the certificate of analysis under aliquotation to the vial of lyophilized powder. Rotate vial gently until solid dissolves. After reconstitution the solution should be cooled on ice for short term storage.
    
    Activity
    
    Add 10 mM Ca2+ to activate His6-rhTG1.
- Applications
  - Application Description
    
    His6-rhTG1 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue. His6-rhTG1 may also be used for immunoprecipitation.
- Reference
  - Chrobok et al., PLoS One. 2018, 13:e0196433
    Plank et al., J Invest Dermatol. 2018, pii: S0022-202X(18)32817-3
    Fischer et al., J. Invest. Dermatol. 2013, 133:1170-7
    Fukui et al., FEBS J. 2013, 280:1420-9
    Schaertl et al., J. Biomol. Screen. 2010, 15:478-87
    Yamane et al., FEBS J. 2010, 277:3564-74