High Binding Streptavidin Coated PCR Plates

Streptavidin High Binding Coated PCR Plates are made in clear polypropylene coated with different molecules for your needs. Their thin walls optimize the heat transfer from the block to the reaction solution. while the rim of the wells is designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates.

· PCR plates are pre blocked with standard proteic blocking (ELISA Blocking)
· Recommended working volume of 50 to 100 μl
· Each coated plate is packed in a single barrier bag with desiccant

Surface:
Streptavidin High Binding coated surface on Streptavidin HB PCR 8 plate offers a powerful and universal instrument for binding any biotinylated molecule (Proteins-Peptides-Polysaccharides-Oligonucleotides-DNA fragments-etc.) finding a special application for those molecules which do not offer reliable bonding by passive adsorption or adsorb in an unfavourable orientation. Unlike the normal streptavidin coated surface, this product is particulary useful to measure biotinylated low molecular weight molecules.

Please contact us at for specific academic pricing.

Background

Streptavidin High Binding Coated Surface offers a powerful and universal instrument for binding any biotinylated molecule, mostly for measuring biotinylated low molecular weight molecules.
Streptavidin High Binding Coated Surface is suitable for binding antibodies or probes for ELISA assays and other target-specific assays. This surface is a powerful and universal instrument for binding any biotinylated molecule (Proteins-Peptides-Polysaccharides-Oligonucleotides-DNA fragments-etc.).

Biotin is a small molecule which can be conjugated to many proteins without loosing or altering their activity, each protein can bind many biotin molecules.

Since each subunit of streptavidin binds one molecule of biotin, the resulting effect is a great increase of the sensitivity of the assay.

Unlike the normal Streptavidin coated surface, these surfaces are particularly useful to measure biotinylated low molecular weight molecules.

· Streptavidin High Binding Coated Surfaces main features
Biomat Streptavidin High Binding Coated products have the following characteristics:
Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Streptavidin High Binding Coated Products ensures:

Uniformity
The Streptavidin High Binding Coated Products show a CV% less than 5 using biotin-HRP as detector and TMB as substrate.

Storage and Stability
The Streptavidin High Binding Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Streptavidin High Binding Coated Microplates Specifications
Coating
Streptavidin High Binding is coated using 100 µl/well.

Binding capacity for small molecules (e.g. d-biotin)
The Streptavidin High Binding coated wells were incubated with biotin solutions, in an ELISA format, using biotinylated peroxidase as detector and TMB as substrate.

Binding capacity for big molecules (e.g. HIgG)
The Streptavidin High Binding coated wells were incubated with biotinylated HIgG in an ELISA format using HIgG-HRP as a detector and TMB as substrate.

· Streptavidin High Binding PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Streptavidin High Binding is coated using 100 µl/tube.

Binding capacity for small molecules (e.g. d-biotin)
Streptavidin High Binding coated PCR tubes strips were incubated with biotin solutions containing biotinylated peroxidase for 30' R.T. After the washing step, the PCR tubes strips were incubated with TMB and blocked with sulphuric acid 1N. The O.D. values were read at 450 nm