Maleimide Coated PCR Plates

Maleimide Coated PCR Plates are made in clear polypropylene coated with different molecules for your needs. Their thin walls optimize the heat transfer from the block to the reaction solution. while the rim of the wells is designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates.

· PCR plates are pre blocked with non proteic blocking buffer
· Recommended working volume of 50 to 100 μl
· Each coated plate is packed in a single barrier bag with desiccant

Surface:
Maleimide coated surface on Maleimide PCR plates offer a powerful instrument for binding biomolecules containing free sulfhydryl groups (e.g. peptides that contains a terminal cysteine or thiols containg haptens) or reducibile disulfide bonds. It's also very useful tool for assay requiring site-directed orientation.

Please contact us at for specific academic pricing.

Background

Maleimide Coated Surface offers a powerful instrument for binding biomolecules containing free sulfhydryl groups or reducible disulfide bonds that are difficult to coat onto polystyrene plates.
Maleimide Coated Surfaces offer a powerful instrument for binding biomolecules containing free sulfhydryl groups (e.g. peptides that contain a terminal cysteine or thiol containing haptens), or reducible disulfide bonds that are difficult to coat onto polystyrene plates. These coated products are a very useful tool for assays requiring site-directed orientation of particular biomolecules especially during antibody production.

At pH 6.5-7.5 maleimide reacts with free sulfhydryl groups to yield stable bonds, while the reaction with amine becomes significant at pH > 7.5.

If sulfhydryl-containing peptides and proteins oxidize in solution and form disulfide bonds, they must be preventively reduced to free sulfhydryls for allowing interaction with maleimide.

· Maleimide Coated Surfaces main features
Biomat Maleimide Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Maleimide Coated Products ensures:

Uniformity
The Maleimide Coated Products show a CV% less than 10 when used as a catcher of Glutathione Ethyl Ester Biotin Amide (BioGEE) in an ELISA format using streptavidin-HRP as detector and TMB as substrate

Storage and Stability
The Maleimide Coated products, if unopened, are stable at 2 – 8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Maleimide Coated Microplates Specifications
Coating
A derived maleimide is coated using 100 μl/well.

Binding capacity
Microplate was saturated with Glutathione Ethyl Ester Biotin Amide (BioGEE) at a concentration of 2.5 µg/ml (250 ng/well) in an ELISA format using Streptavidin-HRP as detector and TMB as substrate.

Maleimide Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
A derived maleimide is coated using 100 μl/tube.