Protein A Coated PCR Plates

Protein A Coated PCR Plates are made in clear polypropylene coated with different molecules for your needs. Their thin walls optimize the heat transfer from the block to the reaction solution. while the rim of the wells is designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates.

· PCR plates are pre blocked with standard proteic blocking (ELISA Blocking)
· Recommended working volume of 50 to 100 μl
· Each coated plate is packed in a single barrier bag with desiccant

Surface:
Protein A coated surface on Protein A PCR Plates is designed for capture IgG applied directly or as antigen/antibody complexes. Among its applications there are: specific and sterically oriented bond of IgG, separation of IgG from other immunoglobulins or contaminants, separation of antigen-antibodies complex and isolation and analysis of fusion proteins.

Please contact us at for specific academic pricing.

Background

Protein A Coated Surface is designed for the capture of IgG applied directly or as an antibody/antigen complex.
Protein A Coated Surface provides an alternative to direct, passive adsorption methods for immobilizing antibodies for ELISA and other plates-based assay techniques.

Protein A specifically binds the Fc region of immunoglobulins of many mammalian species (see Table 1) with an orientation that allows the F(ab)2 binding sites to be freely available for efficient binding to epitope. When coated on a surface, the Protein A can securely capture IgG applied directly or as antigen/antibody complexes.

· Examples of applications for Protein A Coated surface
specific and sterically oriented bond of IgG
separation of IgG from other immunoglobulins
separation of antigen-antibodies complexes
separation of IgG from contaminants
isolation and analysis of fusion proteins
finding and identifying red cell antibodies (only on U-bottom plates) (see Test for finding and identifying red cell antibodies by means of solid phase method)

· Protein A Coated Surfaces main features
Biomat Protein A Coated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only

Also, Protein A Coated Products ensures:

Uniformity
The coated products show a CV% less than 10 when used as a catcher of biotinylated human IgG in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Storage and Stability
The coated products, if unopened, are stable at 2-8°C until the expiration date printed on the label. If opened, store in closed pouch with desiccant and use within the expiration date.

· Protein A Coated Microplates Specifications
Coating
Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 200 µl/well.

Binding capacity
Microplate was saturated with biotinylated human IgG at a concentration of 0.4-0.5 µg/ml (400-500 ng/well) in an ELISA format using Streptavidin-HRP diluted mixed with Streptavidin as detector and TMB as substrate.

Sensitivity
Biotinylated human IgG was detected at a concentration significantly above background in an ELISA format using streptavidin-HRP as detector and TMB as substrate.

Protein A Coated PCR 8 Strip Tubes and PCR Plates Specifications
Coating
Recombinant Protein A (M.W. 38.9 kDa), from Staphylococcus aureus subsp. Aureus, expressed in E. coli, is coated using 100 µl/tube.