Carboxylated PCR Plates

Carboxylated Treated PCR Plates are made in clear polypropylene coated or treated with different molecules for your needs. Their thin walls optimize the heat transfer from the block to the reaction solution, while the rim of the wells is designed to protect against cross-contamination, allowing a reliable seal with the sealing mats tailored to the plates.

Recommended working volume of 50 to 100 μl
Packaging: 5 plates in a transparent bag

Surface:
Carboxylated surface on Carboxylated Treated PCR plates is dedicated to promote the covalent immobilization of compounds containing reactive free amino groups using the EDC mediated amination. This kind of immobilisation can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces such us small peptides (M.W. 1,000-5,000 Da) drugs, toxins or hormones and can provide an oriented immobilisation of molecules in order to secure the integrity and accessibility of their specific sites.

Please contact us at for specific academic pricing.

Background

Carboxylated Surface is dedicated to promote the covalent immobilization of compounds containing reactive free amino groups using the EDC mediated amination.
Surfaces with carboxylic groups covalently bound are dedicated to promote the covalent immobilization of compounds containing reactive free amino groups using the EDC mediated amination.

This kind of immobilization can overcome some of the limitations connected with physical adsorption of the molecules to the surfaces:

immobilization of molecules which are bound weakly or not all by physical adsorption, namely small peptides (M.W. 1,000 – 5,000 Da) drugs, toxin or hormones
oriented immobilization of molecules in order to secure the integrity and accessibility of their specific sites
increased storage stability compared with that of physical adsorption because of the reduced risk of spontaneous desorption
Coupling molecules having an amino group to COOH surface
The amino group presents in any molecules, such as peptides or proteins, binds to Biomat Carboxylated Surface through formation of amide bonds between the amino group presents in the molecule and the surface carboxylic group by the action of carbodiimide.

· Carboxylated Surfaces main features
Biomat Carboxylated products have the following characteristics:

Ready to Use
Manufactured under ISO:9001 guidelines
All lots are tested for uniformity and reproducibility
Certificate of Quality is released for every lot
For Research Use Only
Also, Carboxylated Products ensures:

Storage and Stability
The Carboxylated products are stored at room temperature in sealed PE bags and are stable until the expiration date printed on the label.

Carboxylated Microplates Specifications
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.

· Carboxylated PCR 8 Strip Tubes and PCR Plates Specifications
Treatment
The surface is modified with carboxylic groups.
Volume of treatment 200 µl/well.