Guinea pig liver transglutaminase

Guinea pig liver transglutaminase

Catalog Number:
E001055268ZED
Mfr. No.:
T039
Price:
$791
  • Size:
    10 U
    Quantity:
    Add to Cart:
      • Overview
        • gpTG2-Gene has heen isolated from guinea pig liver cDNA and fused to 6 Histidine-codons at the 5´ end resulting in the N-terminal amino acid sequence MHHHHHHAEDLILE… His6-rgpTG2 is produced in E. coli and purified by ion metal chelating chromatography to more than 95 % purity.

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      • Properties
        • Name
          Tissue-type transglutaminase, TG2, TGase 2, GTGase, gpTG, tissue type protein-glutamine-γ-glutamyltransferase
          Source
          E. coli
          Type
          Recombinant Proteins
          Purification
          > 95 % (visually by SDS-PAGE),
          Formulation
          The purified transglutaminase is lyophilized from 50 mM NaH2PO4, 150 mM NaCl, pH 8. Sample contains maltodextrin.
          Storage
          Store at -20°C. Avoid repeated freezing and thawing. Delivery is possible at ambient temperature
          Note
          INTENDED FOR RESEARCH USE ONLY, NOT FOR USE IN HUMAN, THERAPEUTIC OR DIAGNOSTIC APPLICATIONS.
          Class
          Animal Transglutaminase 2
          Reconstitution
          Add H2O to the vial of lyophilized powder. Rotate vial gently until solid dissolves.
          Activity
          10 mM CaCl2
      • Applications
        • Application Description
          rgpTG2 catalyzes acyl transfer reactions from glutamin residues in proteins or peptides to primary amines, e. g. the formation of ε-(γ-glutamyl) lysine bonds between proteins by transferring the acyl group of a peptide-bound glutamine residue to the primary amino group of a peptide-bound lysine residue.
      • Reference
        • Hauser et al., Amino Acids. 2016, 1-17
          Wodtke et al., Chembiochem. 2016, DOI: 10.1002/cbic.201600048

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