Gentamicin C1a Sulfate, EvoPure®

- Overview
  - Gentamicin C1a Sulfate, EvoPure® is ≥95.0% pure Gentamicin C1a. Gentamicin C1a is part of the Gentamicin C complex, along with Gentamicin C1 and Gentamicin C2, and together this complex makes up 80% of Gentamicin and has the highest antibacterial activity. Gentamicin C1a differs from Gentamicin C1 by a methyl group in the 6’ position of the purpurosamine (2-amino-hexose) ring. The antimicrobial activity of the Gentamicin complex is thought to arise from the lack of hydroxy groups on the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) fragments.
    EvoPure® products have been fully characterized by spectral analysis and are shipped with a comprehensive certificate of analysis containing lot-specific HPLC, MS, HNMR, and FTIR data.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    37713-04-5; 26098-04-4 (free base)
    
    Molecular Formula
    
    C19H39N5O7 · xH2SO4 (lot specific)
    
    Molecular Weight
    
    449.54 g/mol (Free base)
    
    Appearance
    
    White powder
    
    Other Properties
    
    Source: Micromonospora
    PH: 3.5-5.5
    Purity Level: ≥95.0%
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Gentamicin is a broad spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria. It is effective against several strains of Mycoplasma.
    
    Microbiology Applications: Gentamicin EvoPure® compounds can be used to study effects of individual Gentamicin components on various bacterial strains. Representative MIC values include: Bacillis subtilis ATCC 6633: 0.02 µg/mL Escherichia coli ATCC 10536: 1.1µg/mL Staphylococcus aureus ATCC 6538P: 0.06 µg/mL
    Eukaryotic Cell Culture Applications: HIV gene expression is dependent on binding of the viral Tat protein to the transactivation RNA response element. A number of synthetic Tat‐transactivation responsive element interaction inhibitors were described as potential antiviral drug prototypes. Researchers modified commercial Gentamicin C, specifically the purified Gentamicin C1a isomer, by attaching arginine moieties. The result was R4GC1a, a new class of peptidomimetric inhibitors. The cytotoxicity of the compound was studied in LAN 1 (human neuroblastoma), MPC11 (murine plasmacytoma) and MT4 (human T‐lymphocytes) cell cultures. There was no cell growth inhibition in culture using up to 1 mM concentration (Litovchick et al, 1999).