Gentamicin C2 sulfate, EvoPure®

- Overview
  - Gentamicin C2 sulfate, EvoPure® is ≥95.0% pure Gentamicin C2 and can be used to study the Gentamicin biosynthetic pathway. Gentamicin C2 is part of the Gentamicin C complex, along with Gentamicin C1 and Gentamicin C1a, and together this complex makes up 80% of the compound and has the highest antibacterial activity. Gentamicin C2 and C2a are stereoisomers. The higher antimicrobial activity of the complex is thought to arise from the lack of hydroxy groups on the 3’ and 4’ positions of the purpurosamine (2-amino-hexose) fragments.
    EvoPure® products have been fully characterized by spectral analysis and are shipped with a comprehensive certificate of analysis containing lot-specific HPLC, MS, HNMR, and FTIR data.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    25876-11-3 (free base)
    
    Molecular Formula
    
    C20H41N5O7 · xH2SO4 (lot specific)
    
    Molecular Weight
    
    463.57 g/mol (Free base)
    
    Appearance
    
    White or off-white powder
    
    Other Properties
    
    Source: Micromonospora spp.
    PH: 3.5-5.5 (4% in H2O)
    Purity Level: ≥95.0%
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Gentamicin is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria. It is effective against several strains of Mycoplasma.
    
    Microbiology Applications: Gentamicin EvoPure® compounds can be used to study effects of individual Gentamicin components on various bacterial strains. Representative MIC values include: Bacillis subtilis ATCC 6633: 0.02 µg/mL Escherichia coli ATCC 10536: 1.0µg/mL Staphylococcus aureus ATCC 6538P 0.06 µg/mL
    Eukaryotic Cell Culture Applications: LLC-PK1 porcine proximal tubule cells were exposed to various congeners or native Gentamicin from TOKU-E at a concentration of 1 mg/ml. The C2 component of Gentamicin had minimal cytotoxicity and actually reduced the toxicity of the other Gentamicin components when present in the native mixture. This non-nephrotoxic C2 congener was found to be just as potent against Gram-positive and multidrug-resistant Gram-negative bacteria as native Gentamicin or any of the individual components. (Huang et al, 2015).

Datasheet

MSDS

Documents

Susceptibility and Resistance Data

Gentamicin C2 sulfate, EvoPure®

Gentamicin C2 sulfate, EvoPure®

Background

Documents

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