Gentamicin X2 Sulfate, EvoPure®

36889-17-5

C19H38N4O10 · xH2SO4 (lot specific)

482.52 g/mol (Free base)

White powder

Soluble

Source: Micromonospora spp.
PH: 6.0-7.0
Purity Level: ≥98.0%

-20°C

Spectrum: Gentamicin is a broad-spectrum antibiotic targeting a wide variety of Gram-positive and Gram-negative bacteria. It is effective against several strains of Mycoplasma.

Microbiology Applications: Gentamicin EvoPure® compounds can be used to study effects of individual Gentamicin components on various bacterial strains. Aminoglycosides are a widespread and versatile group of bioactive natural products. Most studies on the mechanisms of Gentamicin toxicity have used the commercial mixture, despite evidence that an individual component of the Gentamicin mixture may have lower toxicity. By defining the enzymes involved in the biosynthetic pathway, you could deliver single components. Gentamicin X2 (TOKU-E), a known substrate for C-methylation from G418 catalyzed by the enzyme GenK, may undergo oxidation from an aldehyde, catalyzed by the enzyme GenQ. (Guo et al, 2014).

Eukaryotic Cell Culture Applications: A safer, less nephrotoxic Gentamicin via the use of a single component, or using a single component for semisynthesis of a novel derivative, is an attractive idea. Scientists used Gentamicin A (TOKU-E) and Gentamycin X2 (TOKU-E) to assemble a set of enzymes to separate single Gentamicin C components into pure fractions. LLC-PK1 porcine proximal tubule cells were exposed to various congeners or native Gentamicin at a concentration of 1 mg/ml. The C2 component of Gentamicin had minimal cytotoxicity and actually reduced the toxicity of the other Gentamicin components when present in the native mixture. This non-nephrotoxic C2 congener was found to be just as potent against Gram-positive and multidrug-resistant Gram-negative bacteria as native Gentamicin or any of the individual components. (Huang et al, 2015).