Gentamicin A Sulfate, EvoPure®

- Overview
  - Gentamicin A Sulfate, EvoPure® is ≥99.0% pure Gentamicin A. Gentamicin A Sulfate can be used to study the conversion of Gentamicin A to other congeners, as the Gentamicin biosynthetic pathway is an attractive target for genetic engineering.
    Gentamicin A is a minor component of Gentamicin, along with B, X2 and others. The minor components account for 20% of Gentamicin as opposed to the major components (Gentamicin C1, Gentamicin C1a, and Gentamicin C2) which account for 80% of Gentamicin.
    EvoPure® products have been fully characterized by spectral analysis and are shipped with a comprehensive certificate of analysis containing lot-specific HPLC, MS, HNMR, and FTIR data.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Aminoglycosides target the 30S ribosomal subunit resulting in an inability to read mRNA ultimately producing a faulty or nonexistent protein.
- Properties
  - CAS Number
    
    13291-74-2
    
    Molecular Formula
    
    C18H36N4O10 · xH2SO4 (lot specific)
    
    Molecular Weight
    
    468.50 g/mol (Free base)
    
    Appearance
    
    White powder
    
    Other Properties
    
    Source: Micromonospora spp.
    Purity Level: ≥99.0%
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Gentamicin is a broad-spectrum antibiotic targeting a wide range of Gram-positive and Gram-negative bacteria. Gentamicin has been found to be effective against certain β-lactam sensitive VRE or vancomycin resistant Enterococcus; a glycopeptide antibiotic resistant "superbug."
    
    Microbiology Applications: Gentamicin EvoPure® compounds can be used to study effects of individual Gentamicin components on various bacterial strains.
    
    Eukaryotic Cell Culture Applications: The perspective of a safer, less nephrotoxic Gentamicin by using a single component, or using a single component for semisynthesis of a novel derivative, is a very attractive idea, and scientists studying the molecular enzymology of the pathway in M. echinospora, used Our Gentamicin A and Gentamycin X2 to assemble a defined set of enzymes to separate single Gentamicin C components into pure fractions. LLC-PK1 porcine proximal tubule cells were exposed to various congeners or native Gentamicin at a concentration of 1 mg/ml. The C2 component of Gentamicin had C2 component of Gentamicin had minimal cytotoxicity and actually reduced the toxicity of the other Gentamicin components when present in the native mixture. This non-nephrotoxic C2 congener was found to be just as potent against Gram-positive and multidrug-resistant Gram-negative bacteria as native Gentamicin or any of the individual components (Huang et al, 2015).

MSDS

Gentamicin A Sulfate, EvoPure®

Background

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