Fosfomycin Calcium

- Overview
  - Fosfomycin Calcium is the calcium salt of Fosfomycin, a broad-spectrum phosphonic acid epoxide antibiotic that inhibits bacterial cell wall synthesis. It is used against bacteria causing urinary tract and intestinal infections. It was discovered in Spain in 1969.
    
    Please contact us at for specific academic pricing.
    
    Background
    
    Fosfomycin is a phosphoenolpyruvate analogue that irreversibly inhibits enolpyruvate transferase, preventing the formation of N-acetylmuramic acid, which is an essential element of the peptidoglycan cell wall. It has an extremely low molecular weight, and shows almost no binding to proteins. It is chemically unrelated to any other known antibiotic.
- Properties
  - CAS Number
    
    26469-67-0
    
    Molecular Formula
    
    C3H5CaO4P∙H2O
    
    Molecular Weight
    
    194.12
    
    Appearance
    
    White powder
    
    Other Properties
    
    Source: Streptomyces fradiae. It is also produced by S. viridochromogenes, S. wedmorensis. It may also be produced synthetically.
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Active against Gram-positive and Gram-negative bacteria
    
    Microbiology Applications: Fosfomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive, and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    
    E. coli 0.19 µg/mL – 64 µg/mL
    Helicobacter pylori 0.05 µg/mL – 12.5 µg/mL
    
    AST methods include agar dilution, broth dilution, disk diffusion, and Etest techniques (Falagas et al, 2010).
    
    Eukaryotic Cell Culture Applications: Fosfomycin has immunomodulatory effects, altering lymphocyte, monocyte and neutrophil function. It affects the inflammatory cytokine response in vitro. It can suppress the production of tumor necrosis factor alpha (TNF-a), interleukin-1B and IL-1a. In vitro infection models of biofilm have been used, and Fosfomycin alone or in combination, has the ability to penetrate biofilms.
    Researchers studied chronic biofilm formation by P. aeruginosa due to cystic fibrobris. P. aeruginosa biofilms were grown on cultured human CF-derived airway cells (CFBE41o-). CFU per ml was reduced 4 log10 units when given Fosfomycin in combination with Tobramycin at 256 mg/L. When given at 64 mg/L, initial biofilm formation was inhibited (Anderson et al, 2013).