EZ Cap™ Renilla Luc mRNA (5-moUTP)

Renilla Luc mRNA with Cap 1 structure, modified by 5-moUTP, providing higher transcription efficiency and suppressing RNA-mediated innate immune activation.

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Background

EZ Cap™ Renilla Luc mRNA (5-moUTP) will express Renilla luciferase protein once entering cells, which is initially extracted from enilla reniformis (sea pansy). This enzyme catalyzes Coelenterazine oxidation and lead to yield chemiluminescence at about 480 nm wavelength. Renilla Luc is a frequently used bioluminescent reporter for gene regulation and function study. It is applicable in assays for mRNA delivery, translation efficiency, cell viability and in vivo imaging etc.
EZ Cap™ Renilla Luc mRNA (5-moUTP) is provided at a concentration of 1mg/ml. It is co-transcriptional capped which generates a cap 1 structure with high efficiency. Cap 1 structure is more ideal for mammalian systems and possesses higher transcription efficiency than Cap 0 structure (ARCA and mCap). 5-moUTP and poly (A) tail suppress RNA-mediated innate immune activation and increase the stability and lifetime of the mRNA in vitro and in vivo. Poly (A) tail also plays an important role in enhancing the efficiency of translation initiation.
All the modifications are intention to mimics a fully processed mature mRNA. EZ Cap™ Renilla Luc mRNA (5-moUTP) is an ideal product to observing mRNA delivery, translation and other behaviors.