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Overview
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Selective release of biantennary and high mannose (at a 40X reduced rate) glycans from peptides and proteins.
Endo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.
Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However, for optimal results, denaturation of the glycoprotein is recommended.
Additional Endo F Products:
Endoglycosidase F1 releases bianatennary and some hybrid glycans
Endoglycosidase F3 will release triantennarry and fucosylated biantennary N-glycans
Contents:
Ludger Endoglycosidase F2 - Kit contents
60 µL aliquot of enzyme (0.3 U) in 10 mM sodium acetate 25mM NaCl, pH 4.5
5x Reaction Buffer - 250 mM sodium acetate, pH 4.5Please contact us at for specific academic pricing.
More Details
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