O-glycosidase

O-glycosidase

Catalog Number:
E001088469LUD
Mfr. No.:
E-G001
Price:
$525
  • Size:
    75 mU/60 µL
    Quantity:
    Add to Cart:
      • Overview
        • Cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.

          Specificity:
          O-glycosidase leaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.
          Substitutions such as sialic acid, galactose, fucose or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-O-Glycosidase. At minimum, a sialadase such as Sialadase Au α(2-3,6,8,9), part number E-S001, is almost always required to remove sialic acids.

          Contents:
          60 µL aliquot of enzyme (75 mU) in 50 mM sodium phosphate, pH 7.5.
          5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Source
          Recombinant Streptococcus pneumoniae in E.Coli
          Purification
          O-Glycosidase is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
          Storage
          Store enzyme at 4°C.
          Note
          Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
          Activity
          >1.25 U/mL

          More Information

          Database Link

          * For research use only

      • Applications
        • Application
          Glycan Release
      • Reference
        • 1. Bhavanandan, V.P., J. Umemoto and E.A. Davidson. Characterization of an endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae. Biochem Biophys 70: 738-74 5 (1976 ).
          2. Fan, J.Q., K. Yamamoto, H. Kumagai and T. Tochikura. Induction and efficient purification of endo-alpha-N-acetyl- D-galactosaminidase from Alcaligenes sp. Agric Biol Chem 54: 233-23 4 (1990 ).
          3. Glasgow, L R., J.C. Paulson and R.L. Hill. Systematic purification of five glycosidases from Streptococcus pneumoniae. J Biol Chem 252: 8615-8 623 (1977).
          4. Iwase, H. and K . Hotta. Release of O-linked glycoprotein glycans by endo-alpha-N-acetyl-D-galactosaminidase. Meth Mol Biol 14: 151-159 (1993).
          5. Unemoto, J., V.P. Bhavanandan and E.A. Davidson. Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae. J Biol Chem 252: 8609-8 614 (1977).

    Documents

    Specsheet

    CofA

    MSDS

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