O-glycosidase

- Overview
  - Cleaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.
    
    Specificity:
    O-glycosidase leaves only unsubstituted Gal-β(1-3)GalNAc-α disaccharides attached to the serine or threonine residues of glycoproteins or glycopeptides.
    Substitutions such as sialic acid, galactose, fucose or N-acetylglucosamine must first be removed with the appropriate exoglycosidase prior to treatment with Endo-O-Glycosidase. At minimum, a sialadase such as Sialadase Au α(2-3,6,8,9), part number E-S001, is almost always required to remove sialic acids.
    
    Contents:
    60 µL aliquot of enzyme (75 mU) in 50 mM sodium phosphate, pH 7.5.
    5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Glycosidases
- Properties
  - Source
    
    Recombinant Streptococcus pneumoniae in E.Coli
    
    Purification
    
    O-Glycosidase is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
    
    Activity
    
    >1.25 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.97
    
    * For research use only
- Applications
  - Application
    
    Glycan Release
- Reference
  - 1. Bhavanandan, V.P., J. Umemoto and E.A. Davidson. Characterization of an endo-alpha-N-acetylgalactosaminidase from Diplococcus pneumoniae. Biochem Biophys 70: 738-74 5 (1976 ).
    2. Fan, J.Q., K. Yamamoto, H. Kumagai and T. Tochikura. Induction and efficient purification of endo-alpha-N-acetyl- D-galactosaminidase from Alcaligenes sp. Agric Biol Chem 54: 233-23 4 (1990 ).
    3. Glasgow, L R., J.C. Paulson and R.L. Hill. Systematic purification of five glycosidases from Streptococcus pneumoniae. J Biol Chem 252: 8615-8 623 (1977).
    4. Iwase, H. and K . Hotta. Release of O-linked glycoprotein glycans by endo-alpha-N-acetyl-D-galactosaminidase. Meth Mol Biol 14: 151-159 (1993).
    5. Unemoto, J., V.P. Bhavanandan and E.A. Davidson. Purification and properties of an endo-alpha-N-acetyl-D-galactosaminidase from Diplococcus pneumoniae. J Biol Chem 252: 8609-8 614 (1977).

Documents

Specsheet

CofA

MSDS

O-glycosidase

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Documents

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