Endoglycosidase F3

Endoglycosidase F3

Catalog Number:
E001088467LUD
Mfr. No.:
E-EF03
Price:
$525
  • Size:
    0.33 U/60 µL
    Quantity:
    Add to Cart:
      • Overview
        • Selective release of triantennarry and α(1-6) fucosylated biantennary N-glycans from peptides and protein.

          Endo F3 cleaves free or Asparagine-linked triantennary or α(1-6) fucosylated biantennary oligosaccharides,as well as triamnnosyl chitobiose core structures. Nonfucosylated biantennary glycans will also be cleaved, but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. α(1-3) fucosylation will inhibit enzymatic activity. There is no activity on oligomannose and hybrid molecules.

          Additional Endo F Products
          Endoglycosidase F1 releases bianatennary and some hybrid glycans
          Endoglycosidase F2 will release high mannose and biantennary N-glycans

          Contents:
          Ludger Endoglycosidase F3 - Kit contents
          60 µL aliquot of enzyme (0.33 U) in 20 mM Tris-HCl, pH 7.5
          5x Reaction Buffer - 250 mM sodium acetate, pH 4.5

          Please contact us at for specific academic pricing.

          More Details

      • Properties
        • Name
          Endo F3, Endoglycosidase F3, endo-β-N-acetylglucosaminidase F
          Source
          Recombinant Elizabethkingia miricola in E. Coli
          Purification
          Endoglycosidase F3 is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
          Storage
          Store enzyme at 4°C.
          Note
          Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
          Activity
          >5 U/mL

          More Information

          Database Link

          * For research use only

      • Applications
        • Application
          Glycan Release

    Documents

    Specsheet

    CofA

    MSDS

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