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Overview
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Selective release of triantennarry and α(1-6) fucosylated biantennary N-glycans from peptides and protein.
Endo F3 cleaves free or Asparagine-linked triantennary or α(1-6) fucosylated biantennary oligosaccharides,as well as triamnnosyl chitobiose core structures. Nonfucosylated biantennary glycans will also be cleaved, but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. α(1-3) fucosylation will inhibit enzymatic activity. There is no activity on oligomannose and hybrid molecules.
Additional Endo F Products
Endoglycosidase F1 releases bianatennary and some hybrid glycans
Endoglycosidase F2 will release high mannose and biantennary N-glycans
Contents:
Ludger Endoglycosidase F3 - Kit contents
60 µL aliquot of enzyme (0.33 U) in 20 mM Tris-HCl, pH 7.5
5x Reaction Buffer - 250 mM sodium acetate, pH 4.5Please contact us at for specific academic pricing.
More Details
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- Properties
- Applications
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Overview