Endoglycosidase F1

- Overview
  - Endo F1 cleaves high mannose and some hybrid type N-glycans from peptides and proteins.
    
    Endo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.
    
    Additional Endo F Products:
    Endoglycosidase F2 releases biantennary and high mannose glycans (at a 40X reduced rate)
    Endoglycosidase F3 will release triantennarry and fucosylated biantennary N-glycans
    
    Contents:
    Ludger Endoglycosidase F1 - Kit contents
    60 µL aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5
    5x Reaction Buffer – 250 mM sodium phosphate, pH 5.5
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Glycosidases
- Properties
  - Name
    
    Endo F1, Endoglycosidase F1, endo-β-N-acetylglucosaminidase F
    
    Purification
    
    Endoglycosidase F1 is tested for contaminating protease as follows; 10 µg of denatured BSA is incubated for 24 hours at 37°C with 2 µL of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation.
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient tempertures will not reduce activity.
    
    Activity
    
    >17 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.96
    
    * For research use only
- Applications
  - Application
    
    Glycan Release

Documents

Specsheet

CofA

MSDS

Endoglycosidase F1

More Details

More Information

Documents

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