PROTEOfectene® AB - Protein Transfection Reagent for Antibodies

- Overview
  - PROTEOfectene® AB is a lipid-based formulation which forms noncovalent complexes with antibodies. PROTEOfectene® AB allows antibodies delivered into cells retain structure and function. There is no need for covalent linking: just mix PROTEOfectene® AB with your antibody of interest.
    
    Highlights
    ·High efficiency and broad range of cell types
    ·Mono- and polyclonal antibodies of all isotypes
    ·Ultra-low toxicity: cell vitality is maintained
    ·Full serum compatibility
    ·Efficient, rapid protocol
    
    Sizes
    100 μl: 100 μl Antibody transfection reagent + 100 μl IgG-FITC (100μg/ml)
    250 μl: 250 μl Antibody transfection reagent + 100 μl IgG-FITC (100μg/ml)
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1/2 year (from date of delivery)
    Content: PROTEOfectene® AB, 100 μl IgG (FITC labeled; 100 μg/ml) as positive control
    Assays: Approx. 100 (24 well) or approx. 20 (6 well) per 100 μl reagent
    
    Storage
    
    4℃ PROTEOfectene® AB, ≤–15℃ IgG
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    PROTEOfectene® AB can be used to introduce mono- and polyclonal antibodies of all isotypes.
    Application areas:
    ·Immunostaining in living cells
    ·Proteomics to elucidate complex molecular mechanisms

MSDS

Documents

Product Information and Protocol

Proteofection of A549 cells with PROTEOfectene® AB and fluorescein labeled antibodies directed against the nuclear pore complex. Fluorescent microscopy clearly shows the selective binding of antibodies to nuclear pores.

Proteofection of A549 cells with fluorescein labeled antibodies against giantin using PROTEOfectene® AB. Fluorescent microscopy clearly shows the selective binding of antibodies in the region of the Golgi apparatus.

PROTEOfectene® AB - Protein Transfection Reagent for Antibodies

PROTEOfectene® AB - Protein Transfection Reagent for Antibodies

Documents

Proteofection of A549 cells with PROTEOfectene® AB and fluorescein labeled antibodies directed against the nuclear pore complex. Fluorescent microscopy clearly shows the selective binding of antibodies to nuclear pores.

Proteofection of A549 cells with fluorescein labeled antibodies against giantin using PROTEOfectene® AB. Fluorescent microscopy clearly shows the selective binding of antibodies in the region of the Golgi apparatus.

PROTEOfectene® AB - Protein Transfection Reagent for Antibodies

PROTEOfectene® AB - Protein Transfection Reagent for Antibodies

Documents

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