μ-Transfection Kit VI - Microscopy & Transfection

- Overview
  - The μ-Transfection Kit VI combines high-performance transfection reagents and μ-slides to allow direct high-resolution microscopic of living transfected cells.
    The reagent METAFECTENE® μ enables cell transfection to be performed with both DNA and siRNA, with optimum transfect effiency.
    The μ–Slide VI is designed for highly resolving microscopy and is optimal for experiments both with living cells and for fixation and staining of cells.
    
    Highlights
    ·Simple transfection protocol (no optimization necessary)
    ·DNA and siRNA transfection
    ·Immediate live observation possible
    ·Ideal optical properties for transmitted light and fluorescence microscopy
    ·Cells can be fixed and dyed
    ·Compatible with oil immersion
    
    Sizes
    100 μl: 100 μl METAFECTENE® μ + 10 ml 1 × PBS + 15 Slides
    
    Please contact us at for specific academic pricing.
- Properties
  - Categories
    
    Mammalian Cell Line Transfection Reagent
    
    Other Properties
    
    Shelf Life: 1 year (from date of delivery)
    Content: METAFECTENE® μ, 1 × PBS, μ-Slides VI (6 channels each)
    Assays: 90 per kit
    
    Storage
    
    4℃ PBS, ≤–15℃ METAFECTENE® μ
    
    Shipping
    
    At room temperature
    
    * Only for research purposes in vitro, not intended for human or animal diagnostic, therapeutic or other clinical uses.
- Applications
  - Application
    
    Mammalian Cell Line Transfection
    
    Application Description
    
    Suitable for DNA and siRNA transfection and high-resolution microscopy of living mammalian cells (live cell imaging).
    Application areas:
    ·Tracking the expression and path of a fluorescence-tagged protein through the cell
    ·Monitoring the effect of knockdown of a gene

MSDS

Documents

Product Information and Protocol

High-resolution images of transfection of HepG2 cells with pCMV-GFP using μ-Transfection Kit VI (60× oil-immersion objective)

High-resolution images of transfection of HeLa cells with pCMV-GFP using μ-Transfection Kit VI (20× objective)

μ-Transfection Kit VI - Microscopy & Transfection

μ-Transfection Kit VI - Microscopy & Transfection

Documents

High-resolution images of transfection of HepG2 cells with pCMV-GFP using μ-Transfection Kit VI (60× oil-immersion objective)

High-resolution images of transfection of HeLa cells with pCMV-GFP using μ-Transfection Kit VI (20× objective)

μ-Transfection Kit VI - Microscopy & Transfection

μ-Transfection Kit VI - Microscopy & Transfection

Documents

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