Framycetin Sulfate

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Aminoglycosides target the 30S ribosomal subunit, specifically binding to the 16S rRNA and S12 protein. This results in inability to read mRNA ultimately producing a faulty or nonexistent protein. It also induces misreading of the mRNA template and causes translational frameshift, leading to premature termination and bacterial cell death.
- Properties
  - CAS Number
    
    4146-30-9
    
    Molecular Formula
    
    C23H48N6O17S
    
    Molecular Weight
    
    712.72
    
    Appearance
    
    White powder
    
    Solubility
    
    Soluble in water
    
    Other Properties
    
    Source: Streptomyces fradiae
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Broad-spectrum activity against Gram-positive and Gram-negative bacteria. Ineffective against fungi, viruses and most anaerobic bacteria.
    
    Microbiology Applications: Neomycin can be used for gene selection, via exploiting the resistance gene (NPT II) (Aragão, 2009).
    Neomycin is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-positive and Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative effective ranges include:
    
    Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
    Haemophilus influenzae 1.6 µg/mL – 6.3 µg/mL
    
    Neomycin Sulfate can be used for food testing in TSN agar to select for Clostridium perfringens and inhibit growth of Enterobacteria and Clostridium bifermentans.
    
    Plant Biology Applications: Neomycin is commonly used in negative selections for plants which have been successfully been transformed with a plasmid conferring resistance via Agrobacterium mediated transformation.
    
    Eukaryotic Cell Culture Applications: Neomycin is routinely used to select for cells containing resistance plasmids such as pcDNA3 in common cell lines including AtT-20. For additional information for your cell culture needs, please visit our cell-culture database.