All-Fect In Vivo Kit - pDNA Transfection Reagent for Animal Models

ALL-Fect In Vivo Kit (1 mL ALL-Fect reagent at 5 mg/mL + 1 mL Trans-Booster at 2 mg/mL)

The ALL-Fect In Vivo Kit is a broadly acting transfection reagent combination optimized for pDNA delivery in preclinical animal models. The kit components are based on synthetic amphiphilic polymers tailored primarily for pDNA and other nucleic acids delivery after extensive testing of polymer libraries. The components can undergo multivalent interactions with a nucleic acid, encapsulating it into nanoparticles (100- 200 nm) appropriate for effective cellular uptake. This interaction occurs in aqueous buffer, obviating the need for organic solvents during complex preparation. The ALL-Fect Kit components are non-integrating carriers, so that the genetic make-up of host cells is not altered after the treatment. The transfection reagent has been tested and found effective for plasmid DNA, mRNA and siRNA delivery as well in certain cell types. As with all transfection reagents, formulations of ALL-Fect In Vivo Kit with polynucleotides may need to be optimized for specific applications and transfection conditions.

Benefits
• High transfection efficiency in the presence of serum.
• Effective delivery of pDNA molecules via a simple protocol that is ideal for scale-up and automation.
• Minimal toxicity compared to other commercial reagents, reducing adverse effects on highly sensitive human cells.
• Non-integrating transfection reagent eliminates unwanted effects due to host genome integration.
• Possibility of using the same transfection reagent in animal models, leading to harmonized studies.

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Background

ALL-Fect is a highly effective, broadly-acting transfection reagent for attachment dependent and independent cells. ALL-Fect is a synthetic amphiphilic polymer that is tailored for both siRNA and DNA delivery to a wide range of cells. It is capable of undergoing multivalent interactions with polynucleotides and encapsulating co-incubated polynucleotides into 100-200 nm particles with a net positive charge. The complexation between the polynucleotides and the ALL-Fect occurs in aqueous buffers, obviating the need for organic solvents during preparation. ALL-Fect is a non-integrating carrier, so that the genetic make-up of host cells is not altered after treatment with the transfection reagent. ALL-Fect has been tested and found effective in different types of attachment dependent cells as well as suspension-growing cells. As with all transfection reagents, formulations of ALL-Fect with DNA or RNA may need to be optimized for specific cell types and transfection conditions.