Cefoperazone Sodium

- Overview
  - Cefoperazone Sodium is a third-generation, semi-synthetic, broad-spectrum cephalosporin antibiotic. It is resistant to β-lactamase and is often used to study drug-protein binding such as penicillin binding proteins.
    
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    Background
    
    Like β-lactams, cephalosporins interfere with PBP (penicillin binding protein) activity involved in the final phase of peptidoglycan synthesis. PBP’s are enzymes which catalyze a pentaglycine crosslink between alanine and lysine residues providing additional strength to the cell wall. Without a pentaglycine crosslink, the integrity of the cell wall is severely compromised and ultimately leads to cell lysis and death. Resistance to cephalosporins is commonly due to cells containing plasmid encoded β-lactamases. Cefoperazone however, is largely resistant to β-lactamases produced by resistant cells.
- Properties
  - CAS Number
    
    62893-20-3
    
    Molecular Formula
    
    C25H26N9NaO8S2
    
    Molecular Weight
    
    667.65
    
    Appearance
    
    White or slightly yellow powder
    
    Solubility
    
    Soluble in water (50 mg/mL)
    
    Other Properties
    
    Source: Synthetic
    Water Content (Karl Fischer): ≤5%
    PH: 4.5-6.5
    Assay: (On Dried Basis): 870-1015µg/mg
    
    Storage
    
    -20°C
    
    * For research use only
- Applications
  - Application Description
    
    Spectrum: Cefoperazone is active against Gram-positive and Gram-negative bacteria (ie Pseudomonas aeruginosa).
    
    Microbiology Applications: Cefoperazone is commonly used in clinical in vitro microbiological antimicrobial susceptibility tests (panels, discs, and MIC strips) against Gram-negative microbial isolates. Medical microbiologists use AST results to recommend antibiotic treatment options. Representative MIC values include:
    Pseudomonas aeruginosa 0.5 µg/mL – 64 µg/mL
    Haemophilus influenzae 0.06 µg/mL – 2 µg/mL
    Media Supplements
    Cefoperazone can be used as a selective agent in several types of isolation media:
    Campylobacter Blood-Free Selective Agar - CCDA Selective Supplement
    Campylobacter Agar - Campylobacter Selective Supplement (Karmali)
    Blood Free Campylobacter Agar - CDMN Selective Supplement
    Bolton Broth - Bolton Broth Selective Supplement
    Campylobacter Agar Base - Modified Karmali Selective Supplement
    Bolton Broth - Modified Bolton Broth Selective Supplement