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Overview
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GFAP is a protein encoded by the GFAP gene which is approximately 49, 8 kDa. GFAP is localised to the cytoplasm. It is involved in signalling by ERBB4, the Jak-STAT signalling pathway, neural stem cell differentiation pathways, lineage-specific markers and ERK signalling. It is one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. GFAP is expressed in the cells of the nervous system, kidney, blood, eye and heart. Mutations in the GFAP gene may result in Alexandra disease. STJ96961 was developed from clone 5C8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody binds endogenous GFAP.
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Overview
Western blot analysis of lysates from 1) Rat Brain Tissue, 2) HeLa, 3) A431, 4) PC12 cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) Tubulin Beta Polyclonal Antibody (cat: (STJ96145) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
Immunofluorescence analysis of Rat-brain tissue. 1, GFAP monoclonal antibody (5C8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Western blot analysis of Rat Brain Tissue, diluted at 1:5000.
Immunofluorescence analysis of Mouse-brain tissue. 1, GFAP monoclonal antibody (5C8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, GFAP monoclonal antibody (5C8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1, GFAP monoclonal antibody (5C8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver tissue. 1, GFAP monoclonal antibody (5C8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, AR Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GFAP monoclonal antibody (5C8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-GFAP antibody
Anti-GFAP antibody
Catalog Number:
A001052330STJ
Mfr. No.:
STJ96961
Price:
$224
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