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Overview
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Aquaporin 4 is a protein encoded by the AQP4 gene which is approximately 34, 8 kDa. Aquaporin 4 is localised to the cell membrane. It is involved in aquaporin-mediated transport, vasopressin-regulated water reabsorption and transport of glucose, bile salts, metal ions and amine compounds. This protein falls under the aquaporin family of intrinsic membrane proteins that function as water-selective channels in the plasma membranes of many cells. This protein is the predominant aquaporin found in brain and has an important role in brain water homeostasis. It acts as an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. Aquaporin 4 is expressed in the cells of the nervous system, lung, eye, muscle and blood. Mutations in the AQP4 gene may result in brain oedema. STJ96964 was developed from clone 4H1 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This primary antibody detects endogenous Aquaporin 4 proteins.
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Overview
Western blot analysis of lysates from 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour. (Red) GAPDH Polyclonal Antibody (cat: (STJ97090) antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody (cat: (NA) was diluted at 1:10000, 37°C 1hour.
IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.
Western blot analysis of 1) Hela, 2) Mouse Heart tissue, 3) Rat Heart Tissue, diluted at 1:2000.
Immunofluorescence analysis of Mouse-brain tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunofluorescence analysis of Human-appendix tissue. 1, Aquaporin 4 monoclonal antibody (4H1) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, Aquaporin 4 monoclonal antibody (4H1) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-Aquaporin 4 antibody
Anti-Aquaporin 4 antibody
Catalog Number:
A001052333STJ
Mfr. No.:
STJ96964
Price:
$224
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