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Overview
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CD5 is a protein encoded by the CD5 gene which is approximately 54, 5 kDa. CD5 is localised to the cell membrane. It is involved in B cell development pathways, hematopoietic stem cell differentiation pathways and lineage-specific markers. This protein falls under the scavenger receptor cysteine-rich superfamily. It is a type-I transmembrane glycoprotein found on the surface of thymocytes, T-lymphocytes and a subset of B-lymphocytes and may act as a receptor in regulating T-cell proliferation. CD5 is expressed in the blood, pancreas, lymph nodes, bone marrow and spleen. Mutations in the CD5 gene may result in thymus cancer. STJ96973 was developed from clone 10G8 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. This antibody detects endogenous CD5 proteins.
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Overview
Immunohistochemical analysis of paraffin-embedded Human Tonsil Caricnoma using CD5 Mouse mAb diluted at 1:200.
Immunofluorescence analysis of Mouse-heart tissue. 1, CD5 monoclonal antibody (10G8) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1, CD5 monoclonal antibody (10G8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Mouse-kidney tissue. 1, CD5 monoclonal antibody (10G8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, CD5 monoclonal antibody (10G8) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-CD5 antibody
Anti-CD5 antibody
Catalog Number:
A001052342STJ
Mfr. No.:
STJ96973
Price:
$224
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