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Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed STJ71387 (0.3µg/ml) staining of lysates of cell line A431 (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ABCB5 antibody

Anti-ABCB5 antibody

Catalog Number:

A001043172STJ

Mfr. No.:

STJ71387

Price:

$499

Size:

100 µg

Quantity:

Add to Cart:

- Overview
  - Please contact us at for specific academic pricing.
- Properties
  - Host
    
    goat
    
    Antibody Type
    
    polyclonal
    
    Reactivity
    
    human
    
    Purification
    
    Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
    
    Conjugate
    
    Unconjugated
    
    Formulation
    
    Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
    
    Storage
    
    Store at -20°C and avoid freeze-thaw cycles.
    
    Concentration
    
    0.5 mg/ml
    
    Regulatory Status
    
    RUO
    
    Antigen
    
    Atp-Binding Cassette Sub-Family B Member 5; Abcb5 P-Gp; P-Glycoprotein Abcb5 (Gene ID:340273; UniProt:Q2M3G0)
    
    * For research use only
- Applications
  - Application
    
    ELISA; WB; Flow Cytometry
    
    Application Description
    
    ELISA-antibody detection limit dilution 1:32000.
    WB-Approx 80kDa band observed in lysates of cell line A431. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
    IF-
    FC-Flow cytometric analysis of HepG2 cells. Recommended concentration: 10ug/ml.

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