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Overview
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CD15 is a protein encoded by the FUT4 gene which is approximately 59 kDa. CD15 is localised to the Golgi stack membrane. It is involved in glycosphingolipid biosynthesis, mannose type O-glycan biosynthesis and metabolism. It transfers fucose to N-acetyllactosamine polysaccharides to generate fucosylated carbohydrate structures. It also catalyses the synthesis of the non-sialylated antigen, Lewis x. CD15 is expressed in the blood, bone marrow, lymph node, spleen and nervous system. Mutations in the FUT4 gene may result in liver lymphoma. STJ97007 was developed from clone Q89 and was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen. The antibody detects endogenous CD15 protein.
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Overview
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Human stomach. 1, Antibody was diluted at 1:200 (4°C overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).
Immunohistochemical analysis of paraffin-embedded Rat Brain Tissue using CD 15 Mouse mAb diluted at 1:500.
Immunofluorescence analysis of Human-liver-cancer tissue. 1, CD15 monoclonal antibody (Q89) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Human-lung-cancer tissue. 1, CD15 monoclonal antibody (Q89) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Anti-CD15 antibody
Anti-CD15 antibody
Catalog Number:
A001052376STJ
Mfr. No.:
STJ97007
Price:
$224
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