-
-
Overview
-
Caspase-8 is a protein encoded by the CASP8 gene which is approximately 55, 3 kDa. Caspase-8 is localised to the cytoplasm and is involved in the TNFR1 pathway, dimerization of procaspase-8, activated TLR4 signalling, apoptosis signalling and toll-like receptor signalling pathways. This protein falls under the cysteine-aspartic acid protease family. It pays a role in the programmed cell death induced by Fas and various apoptotic stimuli. Caspase-8 isoform 1, 5 and 7 are expressed in a wide variety of tissues. Mutations in the CASP8 gene may result in a caspase-8 deficiency. STJ97394 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. This polyclonal antibody detects endogenous levels of Caspase-8 protein.
Please contact us at for specific academic pricing.
-
- Properties
- Applications
-
Overview
Immunohistochemical analysis of paraffin-embedded Mouse Spleen Tissue using Caspase-8 monoclonal antibody.
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue using Caspase-8 monoclonal antibody.
Immunofluorescence analysis of Mouse-liver tissue. 1, Caspase-8 monoclonal antibody (2G12) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1, Caspase-8 monoclonal antibody (2G12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1, Caspase-8 monoclonal antibody (2G12) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
Immunofluorescence analysis of Hela cell. 1, ER Alpha Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Caspase-8 monoclonal antibody (2G12) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).
Anti-Caspase-8 antibody
Anti-Caspase-8 antibody
Catalog Number:
A001052762STJ
Mfr. No.:
STJ97394
Price:
$224
Size:
Quantity:
Add to Cart:
.