Alpha-(1,6) core mannosidase

- Overview
  - Core α Mannosidase cleaves unbranched non-reducing terminal mannose, α(1-6) linked to the β-linked core mannose of the conserved mannosylchitobiose core of N-linked oligosaccharides. The presence of fucose linked to the core N-acetylglucosamine has no effect on cleavage.
    
    The enzyme may inhibit other mannosidases, such as α(1-2,3,6) mannosidase (E-AM01), if a noncleavable α(1-6) mannose is present on the substrate. It should therefore always be added subsequent to digestion by other mannosidases.
    
    Contents:
    Core Alpha Mannosidase in 50 mM sodium phosphate, 0.1 mM ZnCl (pH 7.5).
    200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0).
    
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    More Details
    
    Glycosidases
- Properties
  - Name
    
    α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, core α mannosidase, exo-α-mannosidase
    
    Purification
    
    Each lot of α(1-6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
    
    Activity
    
    1 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.24
    
    * For research use only
- Applications
  - Application
    
    Glycan Release

Documents

Specsheet

CofA

MSDS

Alpha-(1,6) core mannosidase

More Details

More Information

Documents

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