Alpha-( 1 -3,4)-fucosidase

- Overview
  - α(1-3,4) Fucosidase cleaves branched non-reducing terminal fucose, linked α(1-3) or α(1-4) to the N-acetylglucosamine of terminal Gal-GlcNAc disaccharide structures. The presence of sialic acid (but not fucose) linked to the galactose will block cleavage.
    
    α(1-3, 4) Fucosidase is useful for:
    - Fucose linkage determination
    - Deglycosylating glycoproteins with Lewis structures
    
    Contents:
    α(1-3,4)-Fucosidase in 20 mM Tris-HCl, 25 mM NaCl,(pH 7.5).
    5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
    
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    More Details
    
    Glycosidases
- Properties
  - Name
    
    α-L-fucoside fucohydrolase, α-L-fucosidase, α-(1-3,4) fucosidase
    
    Source
    
    Isolated from Xanthamonas manihotis
    
    Purification
    
    Each lot of α(1-3,4) Fucosidase is tested for contaminating activities by incubating the enzyme for 24 hours at 37°C with the appropriate substrates; the detection limit of this assay is 5 µU/mL (IUB). A passing lot will have no detectable activity.
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
    
    Activity
    
    0.5 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.51
    
    * For research use only
- Applications
  - Application
    
    Glycan Release

Documents

Specsheet

CofA

MSDS

Alpha-( 1 -3,4)-fucosidase

More Details

More Information

Documents

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