LudgerZyme α1-3,4 Fucosidase Kit

- Overview
  - The enzyme recognises α1-3,4 fucosylated glycans (e.g. Lewis X/A epitopes, including their sialylated counterparts) and hydrolyses terminal α1-3 and α1-4 fucosyl linkages in these substrates without the need to remove sialic acid. Due to this proprietary feature this enzyme can be used to streamline your workflow, making it both cost and time efficient as there is no need to use sialidase enzyme prior to targeting digestion of antennary fucosylation.
    
    Another advantage is that the enzyme can be used for monitoring α1-3,4 fucosylation on free glycans as well as glycoproteins1 which eliminates the need for chemical or enzymatic release of glycans from glycoproteins.
    
    Amount of Enzyme: 50 µL
    Non-sialidase dependant hydrolysis of antennary fucose moieties
    Effective on both glycopeptides and free glycans
    Highly specific (α1-3,4 fucosylated glycans)
    Kit includes enzyme plus reaction buffer.
    Sufficient for up to 50 samples.
    
    Please contact us at for specific academic pricing.
- Properties
  - Name
    
    α-L-fucoside fucohydrolase, α-L-fucosidase, α-(1-3,4) fucosidase
    
    Source
    
    Recombinant protein from Ruminococcus gnavus E1 strain, expressed in Escherichia coli
    
    Storage
    
    Store at 4°C. Do not freeze. Protect from sources of heat and light. When stored correctly, the enzyme should be stable for 12 months from date of purchase. Exposure to ambient temperatures over several days does not result in a reduction of enzymatic activity.
    
    More Information
    
    Database Link
    
    EC 3.2.1.51
    
    * For research use only
- Applications
  - Application Description
    
    This fucosidase can be used to remove α1-3 and α1-4 linked fucose from free glycans and glycoproteins.
- Reference
  - H. Wu, O. Rebello, E.H. Crost, C. D. Owen, S. Walpole, C. Bennati-Granier, D. Ndeh, S. Monaco, T. Hicks, A. Colvile, P.A. Urbanowicz, M.A. Walsh, J. Angulo, D.I.R. Spencer, N. Juge. Fucosidases from the human gut symbiont Ruminococcus gnavus. Cell Mol Life Sci. 2021; 78(2): 675–693

Documents

Product Guide

LudgerZyme α1-3,4 Fucosidase Kit

LudgerZyme α1-3,4 Fucosidase Kit

More Information

Documents

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