Alpha-( 1 -2,3,6)-mannosidase

Name: Alpha-( 1 -2,3,6)-mannosidase
Price: 762.00 USD

Catalog Number:

E001088482LUD

Mfr. No.:

E-AM01

Price:

$762

Size:

0.6 U/60 µL

Quantity:

Add to Cart:

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- Overview
  - α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.
    
    This enzyme is often used in conjunction with Core α(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.
    
    Contents:
    α(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5)
    200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
    
    Please contact us at for specific academic pricing.
    
    More Details
    
    Glycosidases
- Properties
  - Name
    
    α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, α mannosidase, exo-α-mannosidase
    
    Source
    
    Jack Bean
    
    Purification
    
    Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
    
    Activity
    
    >10 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.24
    
    * For research use only
- Applications
  - Application
    
    Glycan Release
- Reference
  - 1. Snaith, S.M., and G.A. Levvy. Purification and Properties of α-D- Mannosidase from Jack-Bean meal. Biochem. J. 110: 633-670 (1968)
    2. Ganesh Kumar, B.S., Pohlrntz, G., Shculte, M., Mormann, M. Siva Kumar, N. Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool. Glycobiology 24(3):252-61 (2014)