Alpha-( 1 -2,3,6)-mannosidase

- Overview
  - α Mannosidase from Jack Bean cleaves α(1-2,3,6)-linked mannose.
    
    This enzyme is often used in conjunction with Core α(1-6) Mannosidase (E-AM02) if a noncleavable core α(1-6) mannose is present on the substrate.
    
    Contents:
    α(1-2,3,6) Mannosidase in 150 mM sodium phosphate, 0.1 mM ZnCl2 pH 7.5. (pH 7.5)
    200 µL 5x Reaction Buffer 5.0 (250 mM sodium phosphate, pH 5.0)
    
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    More Details
    
    Glycosidases
- Properties
  - Name
    
    α-D-mannosidase, p-nitrophenyl-α-mannosidase, α-D-mannopyranosidase, α mannosidase, exo-α-mannosidase
    
    Source
    
    Jack Bean
    
    Purification
    
    Each lot of α(1-2,3,6) mannosidase is tested for contaminating substances by incubating the enzyme for 24 hours at 37°C with substrates indicated in the table below. No detectable activity is evident for any of these potential contaminants. The detection limit of this assay is 5 µU/mL (IUB).
    
    Storage
    
    Store enzyme at 4°C.
    
    Note
    
    Stable at least 12 months when stored properly. Several days exposure to ambient temperatures will not reduce activity.
    
    Activity
    
    >10 U/mL
    
    More Information
    
    Database Link
    
    EC 3.2.1.24
    
    * For research use only
- Applications
  - Application
    
    Glycan Release
- Reference
  - 1. Snaith, S.M., and G.A. Levvy. Purification and Properties of α-D- Mannosidase from Jack-Bean meal. Biochem. J. 110: 633-670 (1968)
    2. Ganesh Kumar, B.S., Pohlrntz, G., Shculte, M., Mormann, M. Siva Kumar, N. Jack bean α-mannosidase: amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool. Glycobiology 24(3):252-61 (2014)

Documents

Specsheet

CofA

MSDS

Alpha-( 1 -2,3,6)-mannosidase

More Details

More Information

Documents

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