Alkaline Phosphatase Labeling Kit-SH

Alkaline Phosphatase Labeling Kit-SH is used mainly for the preparation of alkaline phosphatase-labeled IgG for enzyme immunoassay (EIA) and for the preparation of alkaline phosphatase-labeled antigen for competitive EIA. SH-reactive ALP, a component of this kit, can react with the thiol groups of proteins or other molecules . This kit contains all the reagents necessary for the labeling process, including reducing agent and storage buffer. SH-reactive ALP forms a covalent link with the target molecule. Reducing agent can create free thiol groups in the IgG molecule. The labeling efficiency of the SH-reactive ALP is high enough to eliminate any purification process after labeling when the alkaline phosphatase-labeled IgG is used for EIA. If a high-purity conjugate is required after labeling, simply use an affinity column or a gel-permeation column. When labeling small molecules, excess molecules can be removed by using the filtration tubes included in this kit.

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