Ac-IETD-AFC

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Ac-IETD-AFC is a fluorogenic substrate of caspase-8 and possesses amino aicd sequence IETD which is recognized by caspase-8-mediated proteolysis. It is also a substrate for caspase-3 processing enzyme, caspase-10, and granzyme B. Caspase activity can be quantified by fluorescent detection of free AFC at Ex. = 400 nm and Em. = 505 nm. Ac-IETD-AFC can be used to identify and quantify caspase-8 activity in apoptotic cell lysates and to study events downstream of caspase-8 activation.
    
    1. Mashima T, Naito M, Kataoka S, Kawai H, Tsuruo T. Aspartate-based inhibitor of interleukin-1 beta-converting enzyme prevents antitumor agent-induced apoptosis in human myeloid leukemia U937 cells. Biochem Biophys Res Commun. 1995; 209(3):907-915.
    2. Nicholson DW, Ali A, Thornberry NA, et al. Identification and inhibition of the ICE/CED-3 protease necessary for mammalian apoptosis. Nature. 1995; 376(6535):17-18.
    3. Thornberry NA, Lazebnik Y. Caspases: enemies within. Science. 1998; 281(5381):1312-1316.
- Properties
  - Alternative Name
    
    4-(sec-butyl)-7-(2-carboxyethyl)-10-(1-hydroxyethyl)-2,5,8,11-tetraoxo-13-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-3,6,9,12-tetraazapentadecan-15-oic acid
    
    CAS Number
    
    211990-57-7
    
    Molecular Formula
    
    C31H38F3N5O12
    
    Molecular Weight
    
    729.65
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥73 mg/mL in DMSO; insoluble in H2O; ≥2.84 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CCC(NC(C(NC(C)=O)C(C)CC)=O)C(NC(C(O)C)C(NC(CC(O)=O)C(NC1=CC(O2)=C(C=C1)C(C(F)(F)F)=CC2=O)=O)=O)=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Ac-IETD-AFC

Background

Documents

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