Z-DEVD-AFC

- Overview
  - Please contact us at for specific academic pricing.
    
    Background
    
    Z-DEVD-AFC is a fluorometric substrate for caspase-3 (Km = 9.7µM) and other related caspases. The sequence DEVD is based on PARP cleavage at Asp216 for caspase-3. During apoptosis, caspase-3 cleaves the substrate and release AFC, thus the caspase activity can be calculated by the fluorescent detection of AFC at Ex. = 400 nm and Em. = 505 nm, or by a blue to green shift in fluorescence. Z-DEVD-AFC can be used to identify and quantify caspase-3 activity in apoptotic cell lysates.
    
    1. Jiuru Sun.; Stephen P. Bottomley.; Sharad Kumar.; Phillip I. Bird. Recombinant Caspase-3 Expressed inPichia pastorisIs Fully Activated and Kinetically Indistinguishable from the Native Enzyme. Biochemical and Biophysical Research Communications. 1997, 238 (3), 920-924.
    2. Kieran F. Harvey.; Natasha L. Harvey.; Julie M. Michael.; Gayathri Parasivam.; Nigel Waterhouse.; Emad S. Alnemri?.; Dianne Watters.; Sharad Kumar. Caspase-mediated Cleavage of the Ubiquitin-protein Ligase Nedd4 during Apoptosis. The Journal of Biological Chemistry. 1998, 273 13524-13530.
- Properties
  - Alternative Name
    
    8-(2-carboxyethyl)-5-(carboxymethyl)-11-isopropyl-3,6,9,12-tetraoxo-14-((2-oxo-4-(trifluoromethyl)-2H-chromen-7-yl)carbamoyl)-1-phenyl-2-oxa-4,7,10,13-tetraazahexadecan-16-oic acid
    
    CAS Number
    
    1135416-11-3
    
    Molecular Formula
    
    C36H38F3N5O14
    
    Molecular Weight
    
    821.71
    
    Appearance
    
    A solid
    
    Purity
    
    98.00%
    
    Solubility
    
    ≥82.2 mg/mL in DMSO; insoluble in H2O; ≥2.5 mg/mL in EtOH with ultrasonic
    
    Storage
    
    Store at -20°C
    
    SMILES
    
    O=C(O)CCC(NC(C(NC(OCC1=CC=CC=C1)=O)CC(O)=O)=O)C(NC(C(C)C)C(NC(CC(O)=O)C(NC2=CC(O3)=C(C=C2)C(C(F)(F)F)=CC3=O)=O)=O)=O
    
    * For Research Use Only

Documents

COA (Certificate Of Analysis)

MSDS (Material Safety Data Sheet)

Z-DEVD-AFC

Background

Documents

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