Name: 5-Carboxy-X-rhodamine
Price: Inquiry

- Overview
  - Single isomer of 5(6)-ROX is a fluorescent dye used in RT-PCR methods as an internal reference to determine fluorescence variation that is not associated with the amplification process (plastic of the wells, small differences in concentration or volume, instrument measurements). It produces a constant fluorescence emission signal during the PCR process that is used to normalise the emission produced by the reporter. The fluorescence signal is compatible with that of most reporters. It is used to label the 5’ end of oligonucleotides as a reporter in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. 6-ROX with NHS-activated carboxylic acids reacts with primary amines.
    
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- Properties
  - Categories
    
    Fine Chemicals
    
    CAS Number
    
    216699-35-3
    
    Molecular Formula
    
    C33H30N2O5
    
    Molecular Weight
    
    534.6 g/mol
    
    MDL Number
    
    MFCD28355897
    
    Note
    
    There is no hazardous surcharge associated with this product.
    There is no special packaging charge associated with this product.
    
    * For research use only. Not for human or veterinary use
- Reference
  - Hahn., et al. (2001). Influence of fluorophore dye labels on the migration behavior of polymerase chain reaction - Amplified short tandem repeats during denaturing capillary electrophoresis. Electrophoresis 22(13):2691-700
    Slateva., et al. (2001). HLA-DRB fluorotyping by dark quenching and automated analysis. Tissue Antigens 58:250-254
    Wang, G., et al. (2007). Optimization of 6-carboxy-X-rhodamine concentration forreal-time polymerase chain reaction using molecular beacon chemistry. Canadian journal of Microbiology 53(3):337-463