Uracil-Specific Excision Reagent (1 U/μL)

Uracil-Specific Excision Reagent (abbreviated as USER) is a hybrid enzyme of uracil DNA glycosylase (UDG) and DNA glycosylase-lyase (Endo VIII), which can produce a single nucleotide gap at the uracil position. UDG catalyzes the cleavage of uracil bases to form an AP site (abstract pyrimidine site), but keeps the phosphodiester backbone structure intact. The lyase activity of Endo VIII breaks the phosphodiester bonds at the 3′ and 5′ ends of the abasic site, releasing abasic deoxyribose.

Enzyme activity: 1 U/μL
Inactivation conditions: Incubate at 75°C for 10 min

1) Reaction system:
10 ×Uracil-Specific Excision Reagent Reaction Buffer: 2μL
dsDNA: X (10 pmol)
Uracil-Specific Excision Reagent (1 U/μL): 1μL
ddH2O: To 20μL
2) Reaction conditions: Mix thoroughly and react at 37°C for 30~60 min.

Components: Uracil-Specific Excision Reagent (1 U/μL) (50 μL/250 μL); 10×Uracil-Specific Excision Reagent Reaction Buffer (1.25 mL/1.25 mL)

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