Name: YeaCell™ 1st Strand Synthesis Kit for Single Cell 3'RNA-seq
Price: 427.00 USD

- Overview
  - YeaCell™ 1st Strand Synthesis kit for Single Cell 3' RNA-seq uses Oligo (dT)18 and TSO Primer primers to reverse transcribe single cell transcript genes, which is suitable for microfluidic micro-systems. Its products can be enriched by PCR reaction with universal primers, and then the restriction enzyme digestion library construction kit is used to construct the sequencing library. This product uses a new reverse transcriptase that has undergone multiple point mutations based on M-MLV (RNase H-) Reverse Transcriptase, which has the advantages of high reverse transcription efficiency, low mismatch rate, and high fidelity.
    
    Components: RT buffer (76 μL); RT Enzyme mix (36 μL)
    
    Please contact us at for specific academic pricing.
- Properties
  - Storage
    
    Store at -25~-15°C, valid for 1 year.
    
    Note
    
    Please use RNase-free consumables and clean the experimental area regularly. It is recommended to use Thermo Fisher's RNAZap high-efficiency nucleic acid removal spray to remove RNase contamination.
    Other reagents required for library construction must be prepared by yourself or purchased separately.
    
    * For Research Use Only
- Applications
  - Application Description
    
    Suitable for high-throughput single-cell full-length cDNA synthesis of mammalian or eukaryotic cells without cell walls.
    10 pg~1 μg total RNA with poly (A).
    This product is not suitable for prokaryotic total RNA and degraded RNA, such as FFPE RNA.

YeaCell™ 1st Strand Synthesis Kit for Single Cell 3'RNA-seq

YeaCell™ 1st Strand Synthesis Kit for Single Cell 3'RNA-seq

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