Synthetic Cre Recombinase mRNA (NLS)

Synthetic Cre Recombinase mRNA (NLS)

Catalog Number:
MR01527221CLI
Mfr. No.:
MRNA1016
Price:
$440
  • Size:
    50 μgs
    Quantity:
    Add to Cart:
      • Overview
        • The synthetic Cre Recombinase mRNA (NLS) expresses the Cre recombinase in transfected mammalian cells. The Cre recombinase gene is fused to a nuclear localization sequence (NLS) that can locate the enzyme to the nucleus where DNA recombination happens. The mRNA has a 5′-Cap-1 structure and a 3’-long poly(A) tail. It also has a 100% substitution of the modified nucleotide N1-methylpseudouridine, which enhances the translation and reduces the innate antiviral response to single-stranded mRNA.

          Cre recombinase is a tyrosine recombinase enzyme derived from the P1 bacteriophage. The enzyme uses a topoisomerase I mechanism to carry out site-specific recombination of DNA between loxP sites, which can be located either on the same or on separate pieces of DNA. Microinjection of the synthetic Cre recombinase mRNA induces site-specific recombination of a transgene in mouse oocytes and the success rate of the recombination event is 100%.

          Please contact us at for specific academic pricing.

      • Properties
        • Type
          Genome Editing, Synthetic Messenger RNA (mRNA)
          Formulation
          lyophilized powder from nuclease-free water
          Storage
          -70°C. Avoid freeze/thaw cycle.

          * For in vitro research use only.

    Note: If you don't receive our verification email, do the following:

    Copyright © Amerigo Scientific. All rights reserved.